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Another one of 17 Americans flown home has mild symptoms, the US health department says.
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Both passengers who arrived back in the US on the government charter plane travelled in "biocontainment units out of an abundance of caution", the statement said.
All 17 US citizens on the flight will now be further screened at a medical facility in Nebraska.
They are among more than 90 passengers of the MV Hondius ship, now docked in Spain's Canary Islands, who are being repatriated. Officials say the risk of a major outbreak is very low.
Three passengers - a Dutch couple and a German woman - have died after travelling on the vessel. Two of them are confirmed to have had the virus.
Hantaviruses are usually carried by rodents, but human transmission of the Andes strain - which the World Health Organization (WHO) believes was contracted by some of the Dutch ship's passengers while in South America - is possible.
Symptoms can include fever, extreme fatigue, muscle aches, stomach pain, vomiting, diarrhoea and shortness of breath.
In its statement early on Monday, the US Department of Health and Human Services said that all passengers "will undergo clinical assessment" at the University of Nebraska Medical Center.
Seven other US passengers had already returned and are being monitored in their home states.
A British national who resides in the US was evacuated along with the 17 American passengers.
Meanwhile, WHO head Tedros Adhanom Ghebreyesus warned that the decision by the US not to follow his organisation's guidelines over the hantavirus outbreak "may have risks".
The WHO has recommended 42 days of isolation for those leaving the MV Hondius.
But Dr Jay Bhattacharya, the acting head of the US Centers for Disease Control (CDC) said he did not want to cause public panic, insisting that human-to-human transmission was rare and it should n
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